Digalactosyl-diacylglycerol-deficiency lowers the thermal stability of thylakoid membranes (2024)

Krumova, S. K. B., Laptenok, S., Kovács, L., Toth, T., van Hoek, A., Garab, G. (2010). Digalactosyl-diacylglycerol-deficiency lowers the thermal stability of thylakoid membranes. Photosynthesis Research, 105(3), 229-242. https://doi.org/10.1007/s11120-010-9581-5

Krumova, S.K.B. ; Laptenok, S. ; Kovács, L. et al. / Digalactosyl-diacylglycerol-deficiency lowers the thermal stability of thylakoid membranes. In: Photosynthesis Research. 2010 ; Vol. 105, No. 3. pp. 229-242.

@article{3a1bc777e93e4617b9441e53fc8003bf,

title = "Digalactosyl-diacylglycerol-deficiency lowers the thermal stability of thylakoid membranes",

abstract = "We investigated the effects of digalactosyl-diacylglycerol (DGDG) on the organization and thermal stability of thylakoid membranes, using wild-type Arabidopsis thaliana and the DGDG-deficient mutant, dgd1. Circular-dichroism measurements reveal that DGDG-deficiency hampers the formation of the chirally organized macrodomains containing the main chlorophyll a/b light-harvesting complexes. The mutation also brings about changes in the overall chlorophyll fluorescence lifetimes, measured in whole leaves as well as in isolated thylakoids. As shown by time-resolved measurements, using the lipophylic fluorescence probe Merocyanine 540 (MC540), the altered lipid composition affects the packing of lipids in the thylakoid membranes but, as revealed by flash-induced electrochromic absorbance changes, the membranes retain their ability for energization. Thermal stability measurements revealed more significant differences. The disassembly of the chiral macrodomains around 55°C, the thermal destabilization of photosystem I complex at 61°C as detected by green gel electrophoresis, as well as the sharp drop in the overall chlorophyll fluorescence lifetime above 45°C (values for the wild type—WT) occur at 4–7°C lower temperatures in dgd1. Similar differences are revealed in the temperature dependence of the lipid packing and the membrane permeability: at elevated temperatures MC540 appears to be extruded from the dgd1 membrane bilayer around 35°C, whereas in WT, it remains lipid-bound up to 45°C and dgd1 and WT membranes become leaky around 35 and 45°C, respectively. It is concluded that DGDG plays important roles in the overall organization of thylakoid membranes especially at elevated temperatures.",

keywords = "light-harvesting-complex, excitation-energy transfer, charge separation kinetics, pigment-protein complexes, intact photosystem-i, arabidopsis-thaliana, circular-dichroism, dgd1 mutant, higher-plants, chlorophyll fluorescence",

author = "S.K.B. Krumova and S. Laptenok and L. Kov{\'a}cs and T. Toth and {van Hoek}, A. and G. Garab and {van Amerongen}, H.",

year = "2010",

doi = "10.1007/s11120-010-9581-5",

language = "English",

volume = "105",

pages = "229--242",

journal = "Photosynthesis Research",

issn = "0166-8595",

publisher = "Springer",

number = "3",

}

Krumova, SKB, Laptenok, S, Kovács, L, Toth, T, van Hoek, A, Garab, G 2010, 'Digalactosyl-diacylglycerol-deficiency lowers the thermal stability of thylakoid membranes', Photosynthesis Research, vol. 105, no. 3, pp. 229-242. https://doi.org/10.1007/s11120-010-9581-5

Digalactosyl-diacylglycerol-deficiency lowers the thermal stability of thylakoid membranes. / Krumova, S.K.B.; Laptenok, S.; Kovács, L. et al.
In: Photosynthesis Research, Vol. 105, No. 3, 2010, p. 229-242.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Digalactosyl-diacylglycerol-deficiency lowers the thermal stability of thylakoid membranes

AU - Krumova, S.K.B.

AU - Laptenok, S.

AU - Kovács, L.

AU - Toth, T.

AU - van Hoek, A.

AU - Garab, G.

AU - van Amerongen, H.

PY - 2010

Y1 - 2010

N2 - We investigated the effects of digalactosyl-diacylglycerol (DGDG) on the organization and thermal stability of thylakoid membranes, using wild-type Arabidopsis thaliana and the DGDG-deficient mutant, dgd1. Circular-dichroism measurements reveal that DGDG-deficiency hampers the formation of the chirally organized macrodomains containing the main chlorophyll a/b light-harvesting complexes. The mutation also brings about changes in the overall chlorophyll fluorescence lifetimes, measured in whole leaves as well as in isolated thylakoids. As shown by time-resolved measurements, using the lipophylic fluorescence probe Merocyanine 540 (MC540), the altered lipid composition affects the packing of lipids in the thylakoid membranes but, as revealed by flash-induced electrochromic absorbance changes, the membranes retain their ability for energization. Thermal stability measurements revealed more significant differences. The disassembly of the chiral macrodomains around 55°C, the thermal destabilization of photosystem I complex at 61°C as detected by green gel electrophoresis, as well as the sharp drop in the overall chlorophyll fluorescence lifetime above 45°C (values for the wild type—WT) occur at 4–7°C lower temperatures in dgd1. Similar differences are revealed in the temperature dependence of the lipid packing and the membrane permeability: at elevated temperatures MC540 appears to be extruded from the dgd1 membrane bilayer around 35°C, whereas in WT, it remains lipid-bound up to 45°C and dgd1 and WT membranes become leaky around 35 and 45°C, respectively. It is concluded that DGDG plays important roles in the overall organization of thylakoid membranes especially at elevated temperatures.

AB - We investigated the effects of digalactosyl-diacylglycerol (DGDG) on the organization and thermal stability of thylakoid membranes, using wild-type Arabidopsis thaliana and the DGDG-deficient mutant, dgd1. Circular-dichroism measurements reveal that DGDG-deficiency hampers the formation of the chirally organized macrodomains containing the main chlorophyll a/b light-harvesting complexes. The mutation also brings about changes in the overall chlorophyll fluorescence lifetimes, measured in whole leaves as well as in isolated thylakoids. As shown by time-resolved measurements, using the lipophylic fluorescence probe Merocyanine 540 (MC540), the altered lipid composition affects the packing of lipids in the thylakoid membranes but, as revealed by flash-induced electrochromic absorbance changes, the membranes retain their ability for energization. Thermal stability measurements revealed more significant differences. The disassembly of the chiral macrodomains around 55°C, the thermal destabilization of photosystem I complex at 61°C as detected by green gel electrophoresis, as well as the sharp drop in the overall chlorophyll fluorescence lifetime above 45°C (values for the wild type—WT) occur at 4–7°C lower temperatures in dgd1. Similar differences are revealed in the temperature dependence of the lipid packing and the membrane permeability: at elevated temperatures MC540 appears to be extruded from the dgd1 membrane bilayer around 35°C, whereas in WT, it remains lipid-bound up to 45°C and dgd1 and WT membranes become leaky around 35 and 45°C, respectively. It is concluded that DGDG plays important roles in the overall organization of thylakoid membranes especially at elevated temperatures.

KW - light-harvesting-complex

KW - excitation-energy transfer

KW - charge separation kinetics

KW - pigment-protein complexes

KW - intact photosystem-i

KW - arabidopsis-thaliana

KW - circular-dichroism

KW - dgd1 mutant

KW - higher-plants

KW - chlorophyll fluorescence

U2 - 10.1007/s11120-010-9581-5

DO - 10.1007/s11120-010-9581-5

M3 - Article

SN - 0166-8595

VL - 105

SP - 229

EP - 242

JO - Photosynthesis Research

JF - Photosynthesis Research

IS - 3

ER -

Krumova SKB, Laptenok S, Kovács L, Toth T, van Hoek A, Garab G et al. Digalactosyl-diacylglycerol-deficiency lowers the thermal stability of thylakoid membranes. Photosynthesis Research. 2010;105(3):229-242. doi: 10.1007/s11120-010-9581-5